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Generation of Rejuvenated Antigen-Specific T Cells by Reprogramming to Pluripotency and Redifferentiation
Authors:Toshinobu Nishimura  Shin Kaneko  Ai Kawana-Tachikawa  Yoko Tajima  Haruo Goto  Dayong Zhu  Kaori Nakayama-Hosoya  Shoichi Iriguchi  Yasushi Uemura  Takafumi Shimizu  Naoya Takayama  Daisuke Yamada  Ken Nishimura  Manami Ohtaka  Nobukazu Watanabe  Satoshi Takahashi  Aikichi Iwamoto  Haruhiko Koseki  Hiromitsu Nakauchi
Institution:1. Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan;2. Division of Infectious Diseases, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan;3. Stem Cell Bank, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan;4. Laboratory of Diagnostic Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan;5. Division of Molecular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan;6. Division of Immunology, Aichi Cancer Center Research Institute, 1-1 Kanakoden, Chikusa-ku Nagoya, Aichi 464-8681, Japan;7. Laboratory for Lymphocyte Development, RIKEN Center for Allergy and Immunology, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan;8. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Central 4, Tsukuba, Ibaraki 305-8562, Japan
Abstract:
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  • Highlights? Reprogramming of antigen-specific T cells to generate iPSCs (T-iPSCs) ? Redifferentiation of CD8+ T cells, with original antigen specificity, from T-iPSCs ? Newly differentiated T cells show high proliferation and elongated telomeres ? T cell antigen-specific cytotoxicity is maintained
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