Polarized distribution of GM1-ganglioside in human duodenal absorptive enterocytes as visualized with cholera toxin-gold complex |
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Authors: | Jürgen Roth |
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Institution: | (1) Interdepartmental Electron Microscopy, Biocenter, University of Basle, CH-4056 Basle, Switzerland |
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Abstract: | Cholera toxin bound to particles of colloidal gold was used to investigate by electron microscopy the binding of the toxin in human duodenum. Cholera toxin binding was detected only in the apical (brush border) plasma membrane domain suggesting that the ganglioside GM1 is absent from the basolateral plasma membrane domain. Intracellularly, toxin binding became detectable in thetrans side of the Golgi apparatus. Labeling of endosomes may indicate that the non toxin-occupied GM1-ganglioside becomes internalized. |
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Keywords: | cholera toxin-gold ganglioside G
M1
electron microscopy human duodenum brush border receptor cycling |
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