Chromosome markers in the tetraploid wheat Aegilops ventricosa analysed by in situ hybridization |
| |
| Authors: | D Bardsley A Cuadrado P Jack G Harrison A Castilho J S Heslop-Harrison |
| |
| Institution: | (1) Department of Cell Biology, John Innes Centre, Colney, Norwich, NR4 7UH, UK Fax: +44-1603-456844 E-mail: pat.heslop-harrison@bbsrc.ac.uk, GB;(2) Department of Cellular Biology and Genetics, University of Alcaláde Henares, E-28871 Alcalá de Henares, Madrid, Spain, ES;(3) Plant Breeding International, Maris Lane, Trumpington, Cambridge, CB2 2LQ, UK, GB |
| |
| Abstract: | Three lines of the tetraploid wheat Aegilops ventricosa Tausch (2n=4x=28), which contains good resistance to eyespot, were analysed using fluorescent in situ hybridization. Probes used included
rDNA, cloned repeated sequences from wheat and rye, simple-sequence repeats (SSRs) and total genomic DNA. The banding patterns
produced could be used to distinguish most chromosome arms and will aid in the identification of Ae. ventricosa chromosomes or chromosome segments in breeding programmes. All lines had a single major 18S-25S rDNA site, the nucleolar
organizing region (NOR) in chromosome 5N and several minor sites of 18S-25S rDNA and 5S rDNA. A 1NL.3DL, 1NS.3DS translocation
was identified, and other minor differences were found between the lines.
Received: 11 August 1998 / Accepted: 28 November 1998 |
| |
| Keywords: | Karyotype evolution Genome evolution In situ hybridization Plant breeding Alien genes Triticum ventricosum |
| 本文献已被 SpringerLink 等数据库收录！ |
|
