酵母的不同显色法的研究

在多年采集分离酵母菌的基础上，本文报道其中43属的600多株主要与担子菌有关的酵母及其他酵母的DBB反应，其中某些菌以往尚未测定过，从而广泛比较了担子菌酵母、子囊菌酵母与某些类酵母的区别。此外在方法上，摸索了其他未见报道的重氮盐的显色效果，发现固红B盐对布掷孢酵母(Bulleta Derx)的反应比DBB更灵敏。同时试验了热碱·乙醇处理细胞的快速DBB测试法，用此法测定某些疑难菌株，结果稳定，可大大缩短测试周期，并适用于多毛有气生菌丝的菌类。

AN INVESTIGATION ON DIFFERENT DBB TESTS AND FRB TEST FOR YEASTS

On the basis of a survey of the yeasts in China, the DBB tests of more than 600 yeasts and yeast-like fungi representing 43 genera including basidiomycetous yeasts were assayed. Some of them have not been previously examined.Alkali-ethanol-DBB method was also used in difficult and complicated cases, and for those mucedinous strains.Some other Diazonium Salts were tested for this purpose. We have found that FRB (Fast Red B Salt) gives rapid and unequivocal positive reaction for Bullera Derx producing tangerine pigment.No pigment was observed for any of the ascomycetous yeasts.The proper use of the colour reaction with different Diazonium Salts and methods for yeast taxonomy is discussed.