The time-course of the effects of ethanol on the redox and phosphorylation states of rat liver |
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| Authors: | R. L. Veech R. Guynn D. Veloso |
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| Affiliation: | Laboratory of Clinical Psychopharmacology, Division of Special Mental Health Research, IRP, and National Institute of Alcohol Abuse and Alcoholism, National Institute of Mental Health, Saint Elizabeths Hospital, Washington D. C., 20032, U.S.A. |
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| Abstract: | 1. The time-course of the effects of ethanol administration on the metabolite concentrations, redox states and phosphorylation state was studied in the freeze-clamped liver of starved rats. The response was found to vary with the time after ethanol administration. 2. Administration of ethanol caused an immediate decrease in the [NAD(+)]/[NADH] ratio of both cytoplasm and mitochondria, which persisted over the 30min studied. 3. The free cytoplasmic [NADP(+)]/[NADPH] ratio in liver decreases immediately after ethanol administration but returns nearly to control values after 15min. 4. The cytoplasmic [ATP]/[ADP][HPO(4) (2-)] ratio is elevated 15min after ethanol administration in the starved rat. 5. The rapid and large changes in most metabolite concentrations measured appeared to result from the maintenance of near-equilibrium in a wide interlinked network. 6. Differences between fed and starved rats 15min after ethanol administration were slight. |
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