Molecular chaperons and co-chaperons,Hsp90, RAR1, and SGT1 negatively regulate bacterial wilt disease caused by Ralstonia solanacearum in Nicotiana benthamiana |
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Authors: | Makoto Ito Kouhei Ohnishi Yasufumi Hikichi Akinori Kiba |
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Affiliation: | 1.Laboratory of Plant Pathology and Biotechnology; Faculty of Agriculture; Kochi University; Nankoku, Japan;2.Research Institute of Molecular Genetics; Kochi University; Nankoku, Japan |
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Abstract: | Ralstonia solanacearum is the causal agent of bacterial wilt disease. To better understand the molecular mechanisms involved in interaction between Nicotiana benthamiana and R. solanacearum, we focused on Hsp90, RAR1 and SGT1. Appearances of wilt symptom were significantly suppressed in Hsp90, RAR1 and SGT1-silenced plants compared with control plants. In RAR1-silenced plants, population of R. solanacearum increased in a similar manner to control plants. In contrast, multiplication of R. solanacearum was significantly suppressed in Hsp90 and SGT1-silenced plants. In addition, expression of PR genes were increased in Hsp90 and SGT1-silenced plants challenged with R. solanacearum. Therefore, RAR1 might be required for disease development or suppression of disease tolerance. These results also suggested that Hsp90 and/or SGT1 might play an important role in suppression of plant defenses leading to disease susceptibility and disease development. |
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Keywords: | Hsp90 Nicotiana benthamiana plant immunity required for Mla12 resistance 1 suppressor-of-G2-allele-of-skp1 virus-induced gene silencing |
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