Blue-light dependent ROS formation by Arabidopsis cryptochrome-2 may contribute toward its signaling role |
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Authors: | Nathalie Jourdan Carlos F Martino Mohamed El-Esawi Jacques Witczak Pierre-Etienne Bouchet Alain d'Harlingue Margaret Ahmad |
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Affiliation: | 1.UMR 8256 (B2A) CNRS – UPMC; IBPS; Université Pierre et Marie Curie 9; Paris, France;2.Department of Biomedical Engineering; Florida Institute of Technology; Melbourne, FL USA;3.Botany Department; Faculty of Science; Tanta University; Tanta, Egypt;4.Xavier University; Cincinatti, OH USA |
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Abstract: | Cryptochromes are blue-light absorbing flavoproteins with many important signaling roles in plants, including in de-etiolation, development, and stress response. They interact with downstream signaling partners such as transcription factors and components of the proteasome, and thereby alter regulation of nuclear gene expression in a light dependent manner. In a prior study, it has also been shown that Arabidopsis cry1 activation by blue light results in direct enzymatic conversion of molecular oxygen (O2) to ROS (reactive oxygen species) in vivo leading to cell death in overexpressing lines. Here we extend these observations to show that Atcry2 is translocated from the cytosol to the nucleus in response to blue light illumination, resulting in nuclear accumulation of ROS in expressing insect cell cultures. These observations suggest that ROS formation may represent a novel means of signaling by Atcry2 distinct from, and perhaps complementary to, the currently known mechanism of light-mediated conformational change. |
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Keywords: | cryptochrome light signaling nuclear localization oxidative stress photoreceptor ROS |
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