Functional analysis of an auxin-inducible DNA-binding protein gene |
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| Authors: | Any Bernstein Amanda Mangeon Janice Almeida-Engler Gilbert Engler Marc Van Montagu Gilberto Sachetto-Martins Dulce Eleonora de Oliveira |
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| Institution: | 1.Laboratório de Genômica Funcional e Transdução de Sinal; Departamento de Genética; Universidade Federal do Rio de Janeiro; Brazil;2.UMR 1301 Interactions Biotiques et Santé Végétale; Institut National de la Recherche Agronomique; Sophia Antipolis, France;3.Institute of Plant Biotechnology Outreach; Gent University; Gent-Zwijnaarde, Belgium |
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| Abstract: | Over the past decades, several studies indicate a correlation between the phytohormone auxin and cell division. The molecular players of this signaling pathway are now being uncovered. DNA Binding Protein1 from Arabidopsis (AtDBP1) is an auxin-inducible gene able to bind DNA non-specifically. In this work the tissue-expression pattern of this gene was investigated. Promoter-GUS analysis demonstrated that the AtDBP1 promoter is active in regions exhibiting intense cell division such as meristems and nematode feeding sites. Also, the promoter expression was modulated upon incubation with cell cycle blockers, indicating a potential role in cell division for this gene. Lastly, AtDBP1 antisense plants presented a higher insensitivity to auxin, and interfered negatively with auxin–induced callus formation and reduced apical dominance. |
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| Keywords: | auxin cell cycle developmental gene expression DNA-binding protein1 (DBP1) nematode |
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