常夏石竹耐盐突变体渗透调节的研究

在离体培养条件下利用γ-射线作诱变剂获得耐0.5%、0.7%、1.0%NaCl的突变系,通过对稳定突变系植株叶片渗透剂含量及对渗透势贡献大小的测定表明耐盐突变体叶中K+、游离氨基酸、Na+、脯氨酸的含量高于对照,其中脯氨酸和Na+积累最明显。而叶片中可溶性糖的含量、K+/Na+低于对照。Na+对突变体植株叶片渗透势贡献最大,是最主要的渗透调节剂之一。耐盐突变体植株内存在渗透物质的再分配,叶内有吸钾排钠现象。

Study on the osmotic potential regulation of Dianthus plumarius variants

By the use of γ-rays as mutagens,we abtained 0.5%,0.7%,1.0% NaCl-tolerant variants from the adventitious buds of Dianthus plumarius in vitro. The measure of osmoregulatents of variants leaves and the contribution of each osmoregulatent to osmotic potential indicate:the content of Na+,K+,proline and free amino acid in variant leaves were higher while the solubale sugar and K+/Na+were lower than that of control plants;the accumulation of proline and Na+ was notable. The Na+ has the biggest contribution to the cytoplasm osmotic potential,so it is one of the most important osmoregulatents. There is osmoregultents re-alloction in the variant plants,and there is assimilating-K+ and eliminating-Na+ mechanism in variants leaves.