| Mg2+和SeO2-3对鸡胚软骨细胞受模拟微重力不良影响的拮抗 |
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| 引用本文： | 张旭,李小兵,李生广,江丕栋,林治焕. Mg2+和SeO2-3对鸡胚软骨细胞受模拟微重力不良影响的拮抗[J]. 生物化学与生物物理进展, 2002, 29(3): 398-401 |
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| 作者姓名： | 张旭 李小兵 李生广 江丕栋 林治焕 |
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| 作者单位： | 中国科学院生物物理研究所，北京 100101;中国科学院生物物理研究所，北京 100101;中国科学院生物物理研究所，北京 100101;中国科学院生物物理研究所，北京 100101;中国科学院生物物理研究所，北京 100101 |
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| 基金项目： | 国家高技术“863”计划资助项目(2-7-2-14). |
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| 摘 要： | 在回转模拟微重力条件下，研究了鸡胚负重软骨细胞骨架的微管系统和碱性磷酸酶活性两项指标的变化，以及1 mg/L亚硒酸钠和5 mmol/L Mg2+对这些指标的影响.流式细胞仪对微管含量的测定显示回转后微管蛋白含量的减少，说明微管系统受到不良影响.碱性磷酸酶活性比对照组明显降低，表明模拟微重力能降低软骨细胞的钙化能力.如果在回转前加入SeO2-3和Mg2+，发现SeO2-3可以在一定程度上拮抗模拟微重力引起的微管蛋白及碱性磷酸酶活性改变，而Mg2+基本上可以完全拮抗模拟微重力对这两项指标的不良影响.
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| 关 键 词： | 软骨细胞，模拟微重力，回转器，碱性磷酸酶，微管 |
| 收稿时间： | 2001-08-24 |
| 修稿时间： | 2001-08-24 |
Antagonistic Effect of SeO32- and Mg2+ Against Changes of ALPase Activity and Microtubulin Content Induced by Simulated Microgravity on Chondrocytes |
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| ZHANG Xu,LI Xiao-Bing,LI Sheng-Guang,JIANG Pei-Dong and LIN Zhi-Huan. Antagonistic Effect of SeO32- and Mg2+ Against Changes of ALPase Activity and Microtubulin Content Induced by Simulated Microgravity on Chondrocytes[J]. Progress In Biochemistry and Biophysics, 2002, 29(3): 398-401 |
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| Authors: | ZHANG Xu LI Xiao-Bing LI Sheng-Guang JIANG Pei-Dong LIN Zhi-Huan |
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| Affiliation: | Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Under the simulated microgravity, the microtubulin content and the alkaline phosphatase activity of cultured chicken embryonic chondrocytes reduced remarkably, which indicated that the simulated microgravity induced the changes of microtublar system and the calcification of chondrocytes. The antagonistic effects of Mg2+ and SeO32- against the changes were studied. The results showed that these changes can be partly antagonized by 1 mg/L Na2SeO3 and completely antagonized by 5 mmol/L Mg2+. |
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| Keywords: | chondrocyte simulated microgravity clinostat alkaline phosphatase microtubule |
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