Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans |
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Authors: | Ravi S Kamath Maruxa Martinez-Campos Peder Zipperlen Andrew G Fraser and Julie Ahringer |
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Institution: | (1) Wellcome/CRC Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK; |
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Abstract: | Background In Caenorhabditis elegans, injection of double-stranded RNA (dsRNA) results in the specific inactivation of genes containing homologous sequences, a technique termed RNA-mediated interference (RNAi). It has previously been shown that RNAi can also be achieved by feeding worms Escherichia coli expressing dsRNA corresponding to a specific gene; this mode of dsRNA introduction is conventionally considered to be less efficient than direct injection, however, and has therefore seen limited use, even though it is considerably less labor-intensive. |
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