Highly selective affinity labelling of RNA polymerase B (II) from wheat germ |
| |
| Authors: | K Yagawa M Nakanishi S Hayashi M Kaku Y Ichinose T Itoh A Tomoda Y Yoneyama N Shigematsu |
| |
| Affiliation: | 1. Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 90, USSR;2. Institut für Biochemie der Ludwig-Maximilians-Universität, D-8000 München 2, FRG |
| |
| Abstract: | DNA-dependent RNA polymerase B (II) from wheat germ was modified by incubation with 4-[N-(β-hydroxyethyl)-N-methyl]benzaldehyde esters of AMP, ADP or ATP, followed by reduction with NaBH4. Reaction of the modified enzyme with [-32P]UTP in the presence of various DNA templates led to a highly selective affinity labelling of the subunit with Mr 140000 by covalently linked ApU. Labelling was inhibited by 1μg/ml -amanitin. |
| |
| Keywords: | RNA polymerase B (II) (Wheat germ) Affinity labeling 4-[N-(β-Hydroxyethyl)-N-methylamino]benzaldehyde Dinucleotide synthesis |
| 本文献已被 ScienceDirect 等数据库收录！ |
