| 一种简单高效的食用真菌总RNA提取方法 |
| |
| 引用本文： | 周凯松,薛久刚,常宁,陈畅,张晗星,张长铠.一种简单高效的食用真菌总RNA提取方法[J].遗传,2003,25(6):703-704. |
| |
| 作者姓名： | 周凯松 薛久刚 常宁 陈畅 张晗星 张长铠 |
| |
| 作者单位： | 山东大学生命科学学院微生物技术国家重点实验室，济南 250100
State Key Laboratory of Microbial Technology,Shandong University,Jinan 250100,China |
| |
| 基金项目： | 高等学校博士学科点专项科研基金(2000042212)资助 |
| |
| 摘 要： | 以金针菇为材料，建立了一种适合于富含RNase、多酚、多糖和糖蛋白的食用真菌RNA的提取方法，此方法在高浓度变性剂存在的条件下2次用苯酚-氯仿-异戊醇进行抽提去除DNA、蛋白质，并用异丙醇和乙酸钠选择性沉淀RNA、去除多糖，得到完整、均一的RNA样品。
Abstract:With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols,polymeric carbohydrates and proteoglycans..Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol,sodium acetate were used to precipitate RNA selectively.Pure and intact RNA can be effectively prepared by this method.
|
| 关 键 词： | 食用菌 RNA提取 RNA extracting domestic fungus 金针菇 Key words |
| 文章编号： | 0253-9772(2003)06-0703-02 |
| 修稿时间： | 2002年10月30 |
A Simple and Effective Method for Extracting Total RNA from Domestic Fungus |
| |
| Kai-Song Zhou,Jiu-Gang Xue,Ning Chang,Chang Chen,Han-Xing Zhang,Chang-Kai Zhang.A Simple and Effective Method for Extracting Total RNA from Domestic Fungus[J].Hereditas,2003,25(6):703-704. |
| |
| Authors: | Kai-Song Zhou Jiu-Gang Xue Ning Chang Chang Chen Han-Xing Zhang Chang-Kai Zhang |
| |
| Affiliation: | State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. |
| |
| Abstract: | With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols,polymeric carbohydrates and proteoglycans..Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol,sodium acetate were used to precipitate RNA selectively.Pure and intact RNA can be effectively prepared by this method. |
| |
| Keywords: | domestic fungus RNA extracting Flammulia velutipes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
|
点击此处可从《遗传》下载全文 |
