Biochemical composition of the marine conditioning film: implications for bacterial adhesion

The conditioning film formed on glass panels was analysed for total carbohydrates (CFCHO), total proteins (CFP) and total uronic acids (CFURA). The influence of these compounds on the adhesion of three marine bacterial cultures, Pseudomonas sp. CE-2, Pseudomonas sp. CE-10 and Bacillus sp. SS-10 was also evaluated. One-way analysis of variance suggested a significant increase in the attachment of all three cultures to conditioned glass panels. Moreover, CE-2 (r = 0.874) and CE-10 (r = 0.879) showed a significant positive correlation with CFCHO. Conversely, SS-10 (r = -0.69) showed a significant negative correlation with CFCHO. Backward multiple linear regression analysis indicated that CFCHO were the most predictive component of the conditioning film in explaining bacterial adhesion to the conditioned glass panels.