Impact of histamine treatment and feeding on the hydrolytic enzyme level of Tetrahymena in culture and in conditions of starvation |
| |
| Institution: | 1. Department of Pharmacological & Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, TX 77204-5000, USA;2. Department of Neurology, The First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Shenyang 110001, China;3. Department of Pharmaceutical Sciences, James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, OH 45267, USA;4. Department of Pharmaceutical Sciences, University of Tennessee College of Pharmacy, Memphis, TN 38163, USA;1. Department of Physiology and Pharmacology, Center for Hypertension and Personalized Medicine, University of Toledo College of Medicine and Life Sciences, University of Toledo Health Science Campus, 3000 Transverse Dr., Toledo, OH 43614, USA;2. Neurobiology Laboratory, National Institute of Environmental Health Sciences, 111 TW Alexander Dr., Research Triangle Park, NC 27709, USA;3. Institute for Biomedical Research (BIOMED UCA-CONICET), Faculty of Medical Sciences, Av. Alicia Moreau de Justo 1600, C1107AFF Buenos Aires, Argentina;1. School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Xinxiang 453003, PR China;2. Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, PR China;1. School of Nuclear Science and Engineering, North China Electric Power University, No. 2, Beinong Road, Beijing 102206, China;2. Beijing Key Laboratory of Passive Safety Technology for Nuclear Energy, North China Electric Power University, Beijing 102206, China;3. State Nuclear Power Software Development Center, Beijing 100029, China |
| |
| Abstract: | - 1.1. The unicellular Tetrahymena pyriformis contains and also produces hydrolytic enzymes, such as glucosidase, phosphatase and glucosaminidase.
- 2.2. Return of Tetrahymena to plain medium after treatment with bacteria alone, histamine alone or bacteria plus histamine was equally followed by persistence of the hydrolytic enzyme activity around the control value and an activity increase at about 60 min.
- 3.3. Incubation of Tetrahymena in salt (Losina-Losinsky) solution after the applied treatment accounted for reduction to practically zero of the glucosidase and glucosaminidase activities, whereas the phosphatase activity tended to increase rather than to decrease in both the bacterium-treated and histamine-treated cultures.
- 4.4. The enzyme activity patterns of the Tetrahymena cells pretreated (imprinted) with histamine did not differ from the control pattern either after re-exposure to histamine or after feeding with bacteria, but showed a uniformization of the activity pattern and a considerable decrease in enzyme activity on incubation (starvation) in salt (Losina-Losinsky) solution.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
