Radioimmunological quantification of C21, C19 and C18 steroids in the gonads of Locusta migratoria migratorioides R. and F. |
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| Institution: | 1. College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China;2. CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;4. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;5. CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;6. Zhongke Tonhe (Shandong) Marine Technology Co., Ltd, Dongying 257200, China;1. Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Ibaraki, Japan;2. Department of Entomology, University of California, Riverside, CA, United States |
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| Abstract: | - 1.1. Levels of progesterone, pregnenolone, testosterone, 5α-dihydrotestosterone, estrone and estradiol were measured by radioimmunoassay in purified gonadal extracts of larval and adult male and female Locusta migratoria.
- 2.2. The average steroid contents varied between less than 1 ng to more than 160 ng/g tissue.
- 3.3. Young adults were treated with precocene or ketoconazole in an attempt to influence the steroid contents in gonads.
- 4.4. Ketoconazole treatment had no effect on the steroid contents in gonads whereas precocene treatment resulted in higher contents of androgens.
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