Effects of Echinostoma trivolvis (Trematoda) infection on neutral lipids,sterols, and carotenoids in Helisoma trivolvis (Gastropoda) |
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| Institution: | 1. University of Goettingen, Albrecht-von-Haller-Institute for Plant Sciences, Department of Plant Biochemistry, Goettingen, Germany;2. University of Goettingen, Goettingen Center for Molecular Biosciences (GZMB), Service Unit for Metabolomics and Lipidomics, Goettingen, Germany;3. University of Goettingen, Goettingen Center for Molecular Biosciences (GZMB), Department of Plant Biochemistry, Goettingen, Germany;1. Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Spain;2. Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain;3. Department of Biology, Healthcare and the Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain;4. Department of Biochemistry and Molecular Biomedicine, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain;1. Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UK;2. Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités - UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, Paris, France;3. INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, Versailles, France;4. Agro Paris Tech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, Versailles, France |
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| Abstract: | - 1.1. Histochemical, thin layer and gas-liquid chromatographic studies were done on neutral lipids, sterols and carotenes in the digestive gland-gonad (DGG) complex of Helisoma trivolvis infected with Echinostoma trivolvis vs uninfected DGG.
- 2.2. Hitochemical Oil Red O staining showed the presence of neutral lipids in the redial body wall and in the digestive cells of the DGG.
- 3.3. TLC showed that free sterols and triacylglycerols were major neutral lipid fractions along with lesser amounts of steryl esters and free fatty acids in the DGG of both populations. The percentage composition of all neutral lipid fractions was greater in infected than uninfected DGG.
- 4.4. Infected DGG contained more carotenoid fractions than uninfected DGG, but only beta-carotene was identified from both.
- 5.5. GLC studies showed that the major sterol present in snail DGG was cholesterol (about 70%) along with lesser amounts of stigmasterol, campesterol, beta-sitosterol and desmosterol. No clear cut distinction was seen in sterols from infected vs uninfected DGG.
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