Sialic acid is an essential moiety of mucin as a hydroxyl radical scavenger |
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Authors: | Ogasawara Yuki Namai Tomoyuki Yoshino Fumihiko Lee Masaichi-Chang-il Ishii Kazuyuki |
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Affiliation: | Department of Environmental Biology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. yo@my-pharm.ac.jp |
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Abstract: | In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; however, desialylated BSM lost this potential. Moreover, we estimated the scavenging effects of BSM against .OH generated by UV irradiation of hydrogen peroxide using ESR analysis. Our results indicate that BSM has .OH scavenging ability the and sialic acid in mucin is an essential moiety to scavenge .OH. |
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Keywords: | BSM, bovine submaxillary gland mucin DMB, 1,2-diamino-4,5-methylenedioxybenzene ESR, electron spin resonance DMPO, 5,5-dimethyl-1-pyrroline-N-oxide EDTA, ethylenediaminetetraacetic acid HEPES, N-(2-hydroxyethyl)-piperazine-N′-(2-ethanesulfonic acid) HPLC, high-performance liquid chromatography LC-MS, liquid chromatography-mass spectrometry NANA, N-acetyl neuraminic acid NGNA, N-glycolyl neuraminic acid ROS, reactive oxygen species TEMPOL, 4-hydroxy-2,2,6,6-tetramethyl-piperidine-N-oxide |
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