Adhesion,spreading, and proliferation of cells on protein carpets: Effects of stability of a carpet |
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Authors: | Michal Opas Ewa Dziak |
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Institution: | (1) Department of Anatomy, University of Toronto, Medical Sciences Bldg., M5S 1A8 Toronto, Ontario, Canada |
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Abstract: | Summary In the present report we have investigated the role that the physical properties of substrata play in modulating the effects
which components of extracellular matrix (ECM) exert on adhesion, spreading, and growth of retinal pigmented epithelial cells.
By simple modifications of conditions for protein adsorption on glass we obtained a set of substrata all coated with proteins
of ECM (protein carpets) but with different physical properties. Using these protein carpets we have shown that their stability
(desorption rate) in tissue culture conditions varies according to the technique with which they were prepared. Both semiremovable
and immobilized carpets are stable, whereas removable protein carpets desorb readily. Therefore, the protein concentration
or composition or both may change with time in tissue culture depending on the technique used to prepare the carpet. In addition,
efficacy of cell attachment to given protein may vary depending on whether a technique used to prepare the protein carpet
involves denaturation of the protein. Adherent cells quickly remove (clear) weakly adsorbed protein carpets and it seems that
the carpet removal is a mechanical process. During the carpet removal cells are rounded, which indicates that a spread cell
phenotype normally associated with stress fibers and focal contacts occurs when the substratum is rigid enough to sustain
cell traction. In addition, substrata lacking the rigidity to support the spread phenotype do not support cell proliferation
either. |
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Keywords: | adhesion spreading proliferation basement membrane cytomechanics retinal pigmented epithelium |
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