In-vitro modulation of protein kinase C activity by environmental chemical pollutants |
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| Authors: | R R Shukla P W Albro |
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| Institution: | 1. Biochemistry and Clinical Biochemistry Institute, Catholic University, School of Medicine, L.go F. Vito n.1, 00168 Rome, Italy;2. Department of Chemical Sciences, University of Messina, V.le F. Stagno d''Alcontres 31, 98166 Messina, Italy;3. Virology and Microbiology AOOR Papardo–Piemonte, V.le F. Stagno d''Alcontres, 98166 Messina, Italy;4. Department of Human Sciences, Society and Health, University of Cassino and Southern Lazio, V. S. Angelo, Loc. Folcara, Cassino, FR 03043, Italy;1. Department of Cellular Pharmacology, Graduate School of Medicine, Hokkaido University, Japan;2. Faculty of Veterinary Medicine, Assiut University, Egypt;3. Central Institute of Isotope Science, Hokkaido University, Japan |
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| Abstract: | A number of environmental chemical pollutants have been reported to cause tumors or help in the propagation of tumors in experimental animals. The in-vitro effects of a few chemical contaminants were studied on the histone phosphorylation and 3H Phorbol dibutyrate (PdBu) binding of partially purified Ca2+/phospholipid dependent protein kinase c (PKC) from the brains of Fischer F344 and B6C3F1 mice. The enzyme was prepared by a modified method which gave approximately 75-fold purification. A differential effect of various compounds was observed on the phosphorylation activity and PdBu binding of PKC from rats and mice. The reported tumor promoting ability and effect on protein kinase C activity appeared to be related in the case of the rat enzyme, although causality cannot be inferred. |
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