Interaction of dopamine-beta-monooxygenase with chromaffin granule membrane lipids. |
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| Authors: | A G Pogosyan A S Boyajian M Y Mkrtchyan K G Karagezyan |
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| Institution: | Institute of Molecular Biology, Academy of Sciences of the Armenian Republic, Yerevan. |
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| Abstract: | The interaction between bovine adrenal medullary dopamine-beta-monooxygenase and liposomes from chromaffin granule membrane lipids as a function of pH, lipid and salt concentration was studied by ultracentrifugation. Efficient adsorption of dopamine-beta-monooxygenase to liposomes occurs in the pH range 5.0-6.5 and at low ionic strength. The adsorption was not detected in the case of apoenzyme. The membrane dopamine-beta-monooxygenase forms a complex with liposomes more effective than soluble does. The data obtained lead to certain conclusions about the specificity of complex between the enzyme and liposomes. |
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