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A bioassay for methylated galacturonan on pulp-fiber surfaces
Authors:Hafrén Jonas  Daniel Geoffrey
Institution:(1) Department of Wood Science, Swedish University of Agricultural Sciences, Wood Ultrastructure Research Centre (WURC), Box 7008, SE-750 07 Uppsala, Sweden
Abstract:A colorimetric bioassay has been developed enabling separation of wood pulp qualities based on the quantity of methyl-esterified galacturonan exposed on fiber surfaces. Thermomechanical pulp (TMP) and chemi-TMP (CTMP) had, respectively, 3.1 and 0.7 mgrg galacturonan on the surface per mg wood pulp. The presence of galacturonan on the surface of TMP and CTMP was further confirmed by immunogold localization.
Keywords:bioassay  fiber surface  immunolocalization  mechanical pulp  methylated galacturonan
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