Production of a functional single-chain Fv protein in transgenic tobacco root exudates |
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| Authors: | Hu Xuejun Zhang Zhichao Bao Yongming Liu Wenzhe Gan Qiang An Lijia |
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| Institution: | (1) Department of Bioengineering, Dalian University of Technology, Dalian, 116012, China;(2) Medical Institute, Dalian University, Dalian, 116622, China |
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| Abstract: | Nicotiana tabacum was transformed with a gene encoding anti-PreS1 of hepatitis B surface antigen single-chain Fv antibody (scFv) and bearing an N-terminal endoplasmic reticulum protein signal peptide sequence. The scFv antibody protein was continuously secreted from the transgenic tobacco roots into a simple hydroponic medium at 630 to 760 ng g–1 dry wt root day–1. The antibody was about 2% of the total secreted protein and still possessed antigen-binding activity. |
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| Keywords: | recombinant antibodies root exudates scFv transgenic plants |
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