The amino acid sequence around the active-site cysteine and histidine residues of stem bromelain |
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Authors: | S S Husain and G Lowe |
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Institution: | The Dyson Perrins Laboratory, University of Oxford, Oxford OX1 3QY, U.K. |
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Abstract: | Stem bromelain that had been irreversibly inhibited with 1,3-dibromo2-(14)C]-acetone was reduced with sodium borohydride and carboxymethylated with iodoacetic acid. After digestion with trypsin and alpha-chymotrypsin three radioactive peptides were isolated chromatographically. The amino acid sequences around the cross-linked cysteine and histidine residues were determined and showed a high degree of homology with those around the active-site cysteine and histidine residues of papain and ficin. |
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