An autoradiographic method of detectingA. laidlawii andM. hyorhinis in cell cultures |
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Authors: | Jarmila Keprtova Kvetoslava Jurmanova Vera Spurna Eva Minarova Jirina Hofmanova Mojmir Nebola |
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Institution: | (1) Institute of Biophysics, Czechoslovak Academy of Sciences, 612 65 Brno 12;(2) Veterinary Research Institute, 621 32 Brno 21, Czechoslovakia |
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Abstract: | Summary The autoradiographic investigation of L cells and Chinese hamster cells for the presence of mycoplasmas (A. laidlawii andM. hyorhinis) using uridine/uracil (UdR/U) testing is a rapid and reliable method suitable for the serial checking of even a small number
of cells. It depends on a reduced incorporation of 3H]uridine and an increased uptake of 3H]uracil into the RNA of mycoplasma-infected cells, shown in autoradiograms by the density of the grains and their distribution.
Results obtained by the autoradiographic technique correspond approximately to specific activity values of RNA-infected cells
after the incorporation of 3H]uridine and 3H]uracil. |
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Keywords: | mycoplasmas tissue culture autoradiography |
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