d-Lysine production from l-lysine by successive chemical racemization and microbial asymmetric degradation |
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Authors: | E Takahashi M Furui H Seko T Shibatani |
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Institution: | (1) Pharmaceutical Development Research Laboratory, Tanabe Seiyaku Co. Ltd., 16-89, Kashima-3-chome, Yodogawa-ku, Osaka 532, Japan Fax: +81 6 300 2593, JP;(2) Production Technology Division, Tanabe Seiyaku Co. Ltd., 16-89, Kashima-3-chome, Yodogawa-ku, Osaka 532, Japan, JP |
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Abstract: | In order to develop a practical process for d-lysine production from l-lysine, successive chemical racemization and microbial asymmetric degradation were investigated. The racemization of l-lysine proceeded quantitatively at elevated temperatures. A sample␣of 1000 strains of bacteria, fungi, yeast and actinomyces
were screened for the ability to degrade l-lysine asymmetrically. Microorganisms belonging to the Achromobacter, Agrobacterium, Candida, Comamonas, Flavobacterium, Proteus, Providencia, Pseudomonas and Yarrowia genera exhibited a high l-lysine-degrading activity. Comamonas testosteroni IAM 1048 was determined to be the best strain and used as a biocatalyst for eliminating the l isomer. The degradation rate of l-lysine with C. testosteroni IAM 1048 was influenced by pH, temperature and agitation speed. Under the optimal conditions, the l isomer in a 100-g/l mixture of racemic lysine was completely degraded within 72 h, with 47 g d-lysine/l left in the reaction mixture. Crystalline d-lysine, with a chemical purity greater than 99% and optical purity of 99.9% enantiomeric excess, was obtained at a yield
of 38% from the reaction mixture by simple purification. An engineering analysis of l-lysine racemization and microbial degradation was carried out to establish the basis of process design for d-lysine production.
Received: 24 September 1996 / Received last revision: 8 November 1996 / Accepted: 23 November 1996 |
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