传染性法氏囊病病毒VP3结构蛋白基因在大肠杆菌中的高效表达

传染性法氏囊病病毒（ＩＢＤＶ）是危害养鸡业的重要病原之一，属双股双节段ＲＮＡ病毒科成员，由Ａ和Ｂ两个片段的ｄｓＲＮＡ构成，大小分别约３３００－３４００ｂｐ和２８００－２９００ｂｐ。基因片段Ａ首先编码ＶＰ２－ＶＰ４－ＶＰ３聚合蛋白，然后再断裂成ＶＰ３、...

HIGH LEVEL EXPRESSION OF VP 3 STRUCTURAL PROTEIN GENE OF CHINESE FIELD VIRULENT IBDV IN E.COLI

The VP 3 structural protein gene fragment of chinese IBDV was amplified by RT-PCR,and inserted into the expression vector pGEX-2TK.Restriction enzyme analysis and partial sequence analysis showed that the sequence of foreign gene cloned was the same as that of VP 3 gene reported before.On the other hand,the gene was located in the BamHI/EcoRI site of this vector in the corrected open reading frame.The recombinant plasmid,pGETVP33,could stably express VP 3 as glutathione s-tranferase(GST) fusion protein with high efficiency (up to 34% of the total bacterial soluble protein) in E.coli BL21 through IPTG induction.Chicken incoulatad with the total bacterial protein in oil adjuvant at 14 days of age could produce IBDV-specific antibody as measured by ELISA and were partially protected against challenge with virulent IBDV.