| 人乳头瘤病毒HPV16 E7蛋白基因在沙门氏菌疫苗菌株X4072中的表达 |
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| 引用本文： | 金顺钱 杨晓. 人乳头瘤病毒HPV16 E7蛋白基因在沙门氏菌疫苗菌株X4072中的表达[J]. 病毒学报, 1998, 14(1): 38-44 |
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| 作者姓名： | 金顺钱 杨晓 |
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| 作者单位： | [1]中国医学科学院中国协和医科大学肿瘤研究所 [2]军事医学科学院生物工程研究所 |
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| 摘 要： | 采用ＰＣＲ方法，从ＨＰＶ１６型质粒中分离出ＨＰＶ１６Ｅ７基因片段，用ＥｃｏＲＩ和Ｓａｌｌ双酶解后，定向插入到表达质粒ｐＹＡ３３３２（ａｓｄ＋）的Ｐｔｒｃ启动子下游，构建成ｐＹＡ３３３２－ＨＰＶ１６－Ｅ７重组表达质粒。在大肠杆菌Ｘ６２１２上筛选出重组质粒后，通过中间菌株Ｘ３７３０的转化过渡，将重组质粒导入鼠伤寒沙门氏减毒疫苗菌株Ｘ４０７２（ａｓｄ－），构建成宿主和载体之间具有平衡致死结构的ＨＰＶ１６
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| 关 键 词： | HPV16 E7 鼠伤寒沙门氏菌 疫苗 重组 |
CONSTRUCTION OF A RECOMBINANT VACCINE STRAIN EXPRESSING E7 GENE OF HUMAN PAPILLOMA VIRUS TYPE 16 |
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| Jin Shunqian Lu Shixin Peng Qiong Tan Wen Chen Ruihong Zhou ChunXiao Yang Xiao Huang Cuifen. CONSTRUCTION OF A RECOMBINANT VACCINE STRAIN EXPRESSING E7 GENE OF HUMAN PAPILLOMA VIRUS TYPE 16[J]. Chinese journal of virology, 1998, 14(1): 38-44 |
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| Authors: | Jin Shunqian Lu Shixin Peng Qiong Tan Wen Chen Ruihong Zhou ChunXiao Yang Xiao Huang Cuifen |
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| Affiliation: | Jin Shunqian 1 Lu Shixin 1 Peng Qiong 1 Tan Wen 1 Chen Ruihong 1 Zhou ChunXiao 1 Yang Xiao 2 Huang Cuifen 2 |
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| Abstract: | E.coli the recombinant plasmid pYA3332-HPV16-E7 in which the expression of E7 gene was under control of promoter Ptrc,and then the recombinant plasmid was successfully introduced into avirulent salmonella tyhimurium vaccine strain X4071(asd-) by serial transformation through mediate strain X3730(asd-),thus constructing recombinant HPV 16 vaccine strain with the balanced-lethal host-vector system.The results of SDS-PAGE and Western blot showed this recombinant vaccine strain can stably and continuously express 12.5kD chimeric protein of HPV16 E7 at appropriate level. |
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| Keywords: | HPV16 E7 gene Salmonella tyhimurium Vaccine Recombination |
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