Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells |
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Authors: | Seung-Cheol Choi Wan-Joo Shim Do-Sun Lim |
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Institution: | (1) Department of Cardiology, College of Medicine, Korea University, Seoul, 136-705, Republic of Korea |
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Abstract: | Vectors encoding reporter genes driven by cardiac specific myosin light chain-2v (MLC-2v), endothelial cell-specific Flk1
or Tie2 promoters were constructed. The cardiac differentiation-monitoring vector (pMLC-2v-DsRed), endothelial cell-specific
monitoring vectors (pFlk1-EGFP, pTie2-EGFP) as well as the dual promoter driven-reporter genes (pMLC-2v-DsRed-Flk1-EGFP, pMLC-2v-DsRed-Tie2-EGFP)
were specifically expressed in the Sca-1+ bone marrow mesenchymal stem cells (BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial
cell-specific promoter-driven reporter vectors provide important tools for the study of stem cell fate and differentiation
in vitro and future stem cell therapy for ischemic cardiomyopathy. |
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Keywords: | Cardiomyogenic differentiation Dual promoters Endothelial differentiation Mesenchymal stem cells Reporter genes |
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