A role of cathepsin B1 in polymorphonuclear leukocytes chemotaxis. |
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Authors: | K Kobayashi Y Endo K Matsuda K Tanaka E Misaka |
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Affiliation: | Central Research Laboratories, Sankyo Co., Ltd., Shinagawa-ku, Tokyo, 140, Japan |
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Abstract: | Cathepsin BI1 was purified from rat liver lysosomal fraction by ammonium sulfate fractionation, followed by chromatography on Sephadex G-200 and DEAE-Sephadex. Formation of chemotactic factor for guinea pig polymorphonuclear (PMN) leukocytes was demonstrated when guinea pig serum was incubated with cathepsin BI. This factor formation was dependent on SH-reagent dithiothreitol (DTT) and was maximal at pH 6.0. ZnSO4, an inhibitor of cathepsin BI, inhibited the chemotactic factor formation likewise. |
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