Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis |
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| Authors: | Byoung Sam Ko Dong-Min Kim Byoung Hoon Yoon Suk Bai Hyeon Yong Lee Jung Hoe Kim Il-Chul Kim |
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| Affiliation: | (1) Higher Education Center for Bioregulator Research, Chonnam National University, Gwangju, 500-757, Korea;(2) College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757, Korea;(3) Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea;(4) College of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 368-701, Korea;(5) Present address: Department of Biological Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, Korea; |
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| Abstract: | To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l−1 h−1, respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production. |
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