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Hybrid thermochemical processing: fermentation of pyrolysis-derived bio-oil
Authors:Laura R Jarboe  Zhiyou Wen  DongWon Choi  Robert C Brown
Institution:(1) Chemical and Biological Engineering, Iowa State University, Ames, IA, USA;(2) Food Science and Human Nutrition, Iowa State University, Ames, IA, USA;(3) Biological and Environmental Sciences, Texas A&M University—Commerce, Commerce, TX, USA;(4) Mechanical Engineering, Iowa State University, Ames, IA, USA
Abstract:Thermochemical processing of biomass by fast pyrolysis provides a nonenzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associated sugars are present in the anhydrous form. Metabolic engineering has enabled utilization of the main anhydrosugar, levoglucosan, in workhorse biocatalysts. The second challenge is the fact that bio-oil is rich in microbial inhibitors. Collection of bio-oil in distinct fractions, detoxification of bio-oil prior to fermentation, and increased robustness of the biocatalyst have all proven effective methods for addressing this inhibition.
Keywords:
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