Transgenic potato overproducing L-ascorbic acid resisted an increase in methylglyoxal under salinity stress via maintaining higher reduced glutathione level and glyoxalase enzyme activity |
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Authors: | Upadhyaya Chandrama Prakash Venkatesh Jelli Gururani Mayank Anand Asnin Leonid Sharma Kavita Ajappala Hemavathi Park Se Won |
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Institution: | (1) Department of Molecular Biotechnology, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul, Korea Republic;(2) Present address: Department of Botany, Guru Ghasidas Central University, Bilaspur, 495009, India; |
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Abstract: | Salt-tolerance was studied in transgenic potato. It was conferred by overexpression of ascorbate pathway enzyme (d-galacturonic acid reductase, GalUR). As genetic engineering of the GalUR gene in potato enhances its ascorbic acid content (l-AsA), and subsequently plants suffered minimal oxidative stress-induced damage, we now report on the comprehensive aptness
of this engineering approach for enhanced salt tolerance in transgenic potato (Solanum tuberosum L. cv. Taedong Valley). Potatoes overexpressing GalUR grew and tuberized in continuous presence of 200 mM of NaCl. The transgenic plants maintained a higher reduced to oxidized
glutathione (GSH:GSSG) ratio together with enhanced activity of glutathione dependent antioxidative and glyoxalase enzymes
under salinity stress. The transgenics resisted an increase in methylglyoxal that increased radically in untransformed control
plants under salinity stress. This is the first report of genetic engineering of ascorbate pathway gene in maintaining higher
level of GSH homeostasis along with higher glyoxalase activity inhibiting the accumulation in methylglyoxal (a potent cytotoxic
compound) under salt stress. These results suggested the engineering of ascorbate pathway enzymes as a major step towards
developing salinity tolerant crop plants. |
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