Bioprocessing of agricultural residues to ethanol utilizing a cellulolytic extremophile |
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Authors: | Vasudeo P Zambare Aditya Bhalla Kasiviswanath Muthukumarappan Rajesh K Sani Lew P Christopher |
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Institution: | (1) Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, Rapid City, SD 57701, USA;(2) Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD, USA;(3) Department of Agricultural and Biosystems Engineering, Center for Bioprocessing Research and Development, South Dakota State University, Brookings, SD, USA; |
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Abstract: | A recently discovered thermophilic isolate, Geobacillus sp. R7, was shown to produce a thermostable cellulase with a high hydrolytic potential when grown on extrusion-pretreated
agricultural residues such corn stover and prairie cord grass. At 70°C and 15–20% solids, the thermostable cellulase was able
to partially liquefy solid biomass only after 36 h of hydrolysis time. The hydrolytic capabilities of Geobacillus sp. R7 cellulase were comparable to those of a commercial cellulase. Fermentation of the enzymatic hydrolyzates with Saccharomyces cerevisiae ATCC 24860 produced ethanol yields of 0.45–0.50 g ethanol/g glucose with more than 99% glucose utilization. It was further
demonstrated that Geobacillus sp. R7 can ferment the lignocellulosic substrates to ethanol in a single step that could facilitate the development of a
consolidated bioprocessing as an alternative approach for bioethanol production with outstanding potential for cost reductions. |
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