Comparison of recombinant protein expression in a baculovirus system in insect cells (Sf9) and silkworm |
| |
Authors: | Usami Akihiro Ishiyama Seiji Enomoto Chiaki Okazaki Hironobu Higuchi Keiko Ikeda Mashahiro Yamamoto Takeshi Sugai Mutsumi Ishikawa Yukiko Hosaka Yumiko Koyama Teruyuki Tobita Yoneko Ebihara Syoko Mochizuki Toshiko Asano Yoshimi Nagaya Hidekazu |
| |
Affiliation: | Katakura Industries Co. Ltd, Research Institute of Biological Science, Sayama, Saitama, Japan. |
| |
Abstract: | Using a hybrid baculovirus system, we compared the expression of 45 recombinant proteins from six categories using two models: silkworm (larvae and pupae) and an Sf9 cell line. A total of 45 proteins were successfully expressed; preparation of hybrid baculovirus was unsuccessful for one protein, and two proteins were not expressed. A similar pattern of expression was seen in both silkworm and Sf9 cells, with double and multiple bands found in immunoblotting of the precipitate of both hosts. Degraded proteins were seen only in the silkworm system (particularly in the larvae). Production was more efficient in silkworms; a single silkworm produced about 70 times more protein than 10(6) Sf9 cells in 2 ml of culture medium. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|