Increase in plasma atrial natriuretic polypeptide (ANP) following sodium load in anesthetized rats |
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Authors: | J Kato O Kida T Higa A Sasaki K Kondo A Miyata K Kangawa H Matsuo K Tanaka |
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Institution: | 1. First Department of Internal Medicine Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan;1. Department of Biochemistry, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan |
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Abstract: | To investigate the releasing mechanisms of atrial natriuretic polypeptide (ANP), identical amounts of 5% glucose solution, isotonic (0.9%) or hypertonic (5%) saline were infused intravenously for 5 min (2 ml/min) in anesthetized rats. At the same time, plasma immunoreactive ANP (ir-ANP) was measured using a direct radioimmunoassay. Plasma ir-ANP increased after infusion of 5% glucose solution (P less than 0.01) and isotonic saline (P less than 0.05), and returned rapidly to the basal levels in the recovery period. Plasma ir-ANP increased to a greater degree in the group infused with hypertonic saline than in the other two groups. The major immunoreactive component of increased ir-ANP was identified as alpha-rat ANP, a 28 amino acid residue, by using reverse phase high-performance liquid chromatography. These results suggest that sodium ions may be a stimulating factor of ANP release as well as volume expansion. |
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