大豆种质资源萌发期耐旱性评价

随着全球气候的变化,干旱已成为限制大豆产量的重要环境因素之一,大豆种质资源耐旱性鉴定对大豆耐旱品种的培育及大豆耐旱机理的研究具有重要的意义。本研究首先选择4份耐旱性不同的材料,以PEG-6000作为渗透胁迫剂,研究了大豆种质在0%、10%、15%、16%、18%、20%、30%的PEG-6000溶液的胁迫下,相对发芽率、相对发芽势、相对胚根长度、萌发耐旱指数的变化。结果表明15%~18%PEG-6000溶液是大豆萌发期耐旱鉴定的适宜浓度范围。本研究采用16%PEG-6000溶液对568份大豆资源进行大豆种子萌发期耐旱鉴定,以相对发芽势、相对发芽率、相对胚根长度、相对苗高、萌发耐旱指数、活力指数作为评价指标,应用平均隶属函数评价法对大豆种质萌发期耐旱性进行综合评价,筛选出耐旱种质4份,较耐旱种质18份,中间型种质110份,较敏感种质194份,敏感型种质242份。

Identification and Evaluation of Soybean Germplasm Resources for Drought Tolerance During Germination Stage

With the change of the global climate,the drought tolerance at germination stage of soybean becomes of importance when suffering spring drought.In order to explore an optimal drought stress condition,four soybean accessions with levels of drought tolerance were tested with the concentrations of 0%,10%,15%,16%,18%,20%,30%of PEG-6000 solution simulated different drought conditions.The result showed that 15%~18%of PEG-6000 solution was qualified to simulate drought stress condition at germination stage of soybean.Moreover,16%of PEG-6000 solution was applied to culture the seeds of 568 soybean germplasm accessions.The indexes were estimated using six indicators including relative germination energy,relative germination rate,relative radical length,relative bud length,germination drought tolerant index and vigor index.Based on subordination function method and the standard classification of drought tolerance in soybean,four drought tolerant varieties,18 medium tolerant varieties,110 intermediate type varieties,194 relatively drought sensitive varieties and 242 drought sensitive varieties were identified.