泛基因组及其在植物功能基因组学研究中的应用

参考基因组是现代功能基因组学的核心框架,以此为基础的现代基因组学技术在过去20年对植物遗传变异发掘、功能基因克隆等研究起了巨大的推动作用.然而,越来越多的研究发现,单一或少数参考基因组不能完整代表和呈现物种或特定群体内的所有基因组变异,因此其在功能基因组学研究中应用存在很大的局限性,甚至会导致错误的结果.泛基因组是指物...

Research Progress on Pangenome and Its Application in Plant Functional Genetics

The reference genome provides a fundamental framework in the field of modern functional genomics.Modern genomic techniques based on reference genomes is the driving force for identification of genetic variations and functional gene cloning in the past two decades.However,with the progress of plant genome research,it is becoming visible that single reference genome cannot represent all the genomic variances within a species or a population.This consequence has become one of the limits for the functional genomic studies.Pangenome refers to the full complement of genes/genomic sequences within a population.By capturing and presenting all the genes/genomic sequences within a population,pangenome can be used as reference genome in functional genetic studies,to overcome the limit posed by single reference genome.While implementing in the state-of-art genomic techniques,pangenome can greatly improve the efficiency and precision of identification of genetic variations in plant germplasm,thus becoming one of the frontiers and hotspots in genomic research.In this review,we summarized the definition and recent progress in studies of pangenome,the techniques and strategies applied in pangenome assembly and construction,as well as the application of pangenome in plant genomic and molecular breeding.The current problems and future perspectives of plant pangenome were outlined.This review is expected to provide a better understanding and reference for future study and application of plant pangenome.