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| 大花美人蕉茎尖组织培养技术研究 | |
| 引用本文： | 王丹,雷江丽,吴燕民,吕慧,郁继华.大花美人蕉茎尖组织培养技术研究[J].亚热带植物科学,2008,37(1):33-36. |
| 作者姓名： | 王丹 雷江丽 吴燕民 吕慧 郁继华 |
| 作者单位： | 1. 甘肃农业大学农学院, 甘肃 兰州 730070; 2. 深圳市园林科学研究所, 广东 深圳 518003; 3. 中国农业科学院生物技术研究所, 北京 100081 |
| 基金项目： | 广东省深圳市科技局科研项目 |
| 摘 要： | 以大花美人蕉(Canna×generalis)根茎茎尖为外植体进行组织培养技术研究,筛选出芽诱导适宜的培养基为MS+6-BA 8.0mg/L (单位下同)+TDZ 0.03;MS+6-BA 8.0+TDZ 0.03+NAA 0.1培养基能较好地诱导分化出丛生芽,继代增殖培养中与MS+6-BA 3.0+TDZ 0.03+NAA 0.1培养基交替使用可减少畸形芽,增殖系数达1.67;适宜的生根培养基为MS+6-BA 1.0+NAA 0.5,生根率达66.67%,且植株生长健壮,移栽易成活。 |
| 关 键 词： | 大花美人蕉 茎尖 组织培养 |
| 文章编号： | 1009-7791(2008)01-0033-04 |
| 收稿时间： | 2007-10-25 |
| 修稿时间： | 2007年10月25 |
Research on Shoot-tip Culture of Canna×generalis |
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| WANG Dan,LEI Jiang-li,WU Yan-min,L Hui,YU Ji-hua.Research on Shoot-tip Culture of Canna×generalis[J].Subtropical Plant Science,2008,37(1):33-36. | |
| Authors: | WANG Dan LEI Jiang-li WU Yan-min L Hui YU Ji-hua |
| Institution: | 1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu China; 2. Shenzhen Institute of Landscape Gardening, Shenzhen 518003, Guangdong China; 3. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| Abstract: | The paper mainly studied on tissue culture of Canna×generalis with the stem tips as explants. The results showed that the bud inoculation medium was MS+6-BA 8.0mg/L+ TDZ 0.03mg/L; the best of clump shoot induction and differentiation medium was MS+6-BA 8.0mg/L+TDZ 0.03mg/L+NAA 0.1mg/L; using MS+6-BA 3.0mg/L+TDZ 0.03mg/L+NAA 0.1mg/L as proliferation medium, an optimal proliferation rate was obtained. When the two kinds of medium used alternatively, the effect was better. The optimum rooting medium was MS+6-BA 1.0mg/L+NAA 0.5mg/L, the rate of rooting could reach 66.67%, and cultured in this medium, the plant grew well and easy to survival. |
| Keywords: | Canna× generalis shoot-tip tissue culture |
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