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Escherichia coli RNA polymerase binding and initiation of transcription on fragments of lambda rifd 18 DNA containing promoters for lambda genes and for rrnB, tufB, rplC,A, rplJ,L, and rpoB,C genes. |
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| Authors: | W E Taylor R R Burgess |
| Affiliation: | McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI, 53706 U.S.A. |
| Abstract: | |
| Keywords: | filter binding assay 5′ mRNA base sequence physical mapping b.p. base pairs BSA bovine serum albumin DTT dithiothreitol E/D enzyme-to-DNA weight ratio %Λ unit 480 b.p. (Vollenweider and Szybalski, 1978) PBB Polymerase Binding Buffer PFB Polymerase Filter Buffer NTPs nucleoside triphosphates TEMED |
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