Rapid evaluation in Escherichia coli of antisense RNAs and ribozymes |
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Authors: | C. Tatout E. Gauthier H. Pinon |
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Affiliation: | Centre de Génétique Moléculaire et Cellulaire, UniversitéClaude Bernard, Villeurbanne Cedex, France |
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Abstract: | The characterization of a simple bacterial system using commercially available plasmids and strains, developed to assess the effectiveness of trans- acting (antisense RNA and ribozymes) RNA in Escherichia coli , is reported. This system was used to test various trans- acting RNA molecules against the expression of the I factor, a functional transposable element from Drosophila melanogaster . For this target, results indicate that antisense RNA efficiency depends on the hybridization length between sense and antisense RNA. The introduction of a single hammerhead ribozyme within the antisense RNAs does not increase its inhibitory activity. These predictions were subsequently confirmed in Drosophila melanogaster . |
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