Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal direction |
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| Authors: | Florian B?hrnsen Ulrich Lindner Markus Meier Abdelalim Gadallah Peter Schlenke Hendrik Lehnert Jürgen Rohwedel Jan Kramer |
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| Affiliation: | (1) Institute of Virology and Cell Biology, University of L?beck, Ratzeburger Allee 160, 23538 L?beck, Germany;(2) Institute for Transfusion Medicine and Transplantation Immunology, University of M?nster, Domagkstra?e 11, 48149 M?nster, Germany;(3) Medical Department I, University of L?beck, Ratzeburger Allee 160, 23538 L?beck, Germany |
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| Abstract: | Background Because specific marker molecules for phenotypical identification of mesenchymal stem and progenitor cells are missing, the assessment of the in vitro-differentiation capacity is a prerequisite to characterize these cells. However, classical differentiation protocols are often cell-consuming and time intensive. Therefore, the establishment of novel strategies for differentiation is one topic of current efforts in stem cell biology. The goal of this study was to demonstrate the practicability of a new differentiation test using plastic adherent cell isolates from different tissues. |
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