In vitro production of solasodine from Solanum trilobatum |
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| Authors: | K. Anirudhan and A. S. Nair |
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| Affiliation: | (1) Centro de Investigación Biomédica del Sur, IMSS, Argentina 1, Xochitepec, Mor., México;(2) Centro de Investigación en Biotecnología, UAEM, Av.Universidad 1001, Cuernavaca, Mor., México;(3) Depto.de Biotecnología, CINVESTAV-, Zacatenco, México D.F;(4) Grupo de Biotecnología y Patología Experimental. CIATEJ, Guadalajara,Jal, México;(5) Instituto de Biotecnología UNAM, Cuernavaca, Mor., México |
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| Abstract: | Sucrose concentration in the culture medium affected chlorophyll content, trichome development and amount of solasodine in regenerated plantlets of Solanum trilobatum. High chlorophyll content and glandular trichomes were observed in the plants grown on Murashige and Skoog basal medium supplemented with 131.85 mM sucrose. The solasodine was quantified using reverse phase high performance liquid chromatography. The plantlets cultivated on this medium yielded 35.97 mg g−1 (d.m.) solasodine whereas the field plants used as control yielded only 2.32 mg g−1 (d.m.) of solasodine. |
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