Isolation and characterization of microsatellite loci in the Cape fynbos heath Erica coccinea (Ericaceae) |
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Authors: | J G Segarra-Moragues S Donat-Caerols F Ojeda |
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Institution: | 1. ARAID-UZ, Departamento de Agricultura y Economía Agraria, Escuela Politécnica Superior de Huesca, Universidad de Zaragoza, C/Carretera de Cuarte s/n, E-22071, Huesca, Spain 2. Centro de Investigaciones sobre Desertificación (CIDE, CSIC-UV-GV), Camí de la Marjal s/n, E-46470, Albal, Valencia, Spain 3. Departamento de Biología, Universidad de Cádiz, Campus Río San Pedro, E-11510, Puerto Real, Cádiz, Spain
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Abstract: | Enriched genomic libraries were used to isolate and characterize dinucleotide microsatellite loci in Erica coccinea, a South African Cape fynbos heath species with distinct resprouter and seeder populations. Microsatellites were required
to investigate the effect of the contrasting demographic pattern driven by these two post-fire responses in the population
genetic structure of seeder and resprouter forms within this species. Eight microsatellite loci were characterised and amplified
a total of 106 alleles in 2 samples each of 30 individuals from 1 resprouter and 1 seeder population. Mean allele numbers
were 7.88 and 11.0 for the resprouter and seeder population, respectively. Both populations showed similar average observed
and expected heterozygosity levels, H
O(resprouter) = 0.683, H
O(seeder) = 0.696; H
E(resprouter) = 0.726, H
E(seeder) = 0.756, and average positive inbreeding coefficients F
IS(resprouter) = 0.058, F
IS(seeder) = 0.080. This set of microsatellite loci will be used to conduct a population genetic survey of seeder and resprouter
populations throughout the range of the species. Cross-species transferability was also assayed in four other South African
and four European species of the genus Erica, supporting their potential use for population genetic analyses. |
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Keywords: | |
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