Effect of Antimicrobial Peptides Divergicin M35 and Nisin A on Listeria monocytogenes LSD530 Potassium Channels |
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Authors: | Karim Naghmouchi Djamel Drider Riadh Hammami Ismail Fliss |
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Affiliation: | (1) STELA Dairy Research Center, Nutraceuticals and Functional Foods Institute, Université Laval, G1K 7P4 Québec, QC, Canada;(2) UMR INRA 1014 SECALIM, ENITIAA, ENVN, INRA, Rue de la Géraudière, BP82225, 44322 Nantes Cedex, France;(3) UMR CNRS 8576, Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille, Villeneuve d’Ascq Cedex, France;(4) Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada, T1J 4B1 |
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Abstract: | The aim of this work was to study the effect of antimicrobial peptides: divergicin M35 and nisin A on Listeria monocytogenes LSD 530 potassium (K+) channels: ATP-sensitive (KATP), calcium-activated (BKCa), and depolarization-activated (Kv) types. Increase on K+ efflux and inhibition of cellular growth were observed after adding K+ channel activators pinacidil, NS1619, and cromakalim to divergicin M35. Increase in K+ efflux from log-phase cells was about 18 ± 1.1, 11 ± 0.63, and nmol mg−1 of cell dry weight (CDW) for pinacidil and NS1619, respectively, over the efflux obtained with divergicin M35 alone. Increases in K+ efflux obtained by adding the same K+ channel activators to nisin A fit a completely different profile. Divergicin M35 activates K+ channels, particularly of the Kv and BKCa types and to a lesser extent the KATP type, causing K+ efflux and consequently cell death. |
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