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Changes in Soluble Proteins in Spring Wheat Stressed with Sodium Chloride
Authors:M Ashraf  JW O'Leary
Institution:(1) Institute of Pure and Applied Biology, B.Z. University, Multan, Pakistan;(2) Department of Plant Sciences, College of Agriculture, University of Arizona, Tuczon, AZ 85721, USA
Abstract:Two newly developed salt-tolerant genotypes of spring wheat, S24 and S36 and their salt-tolerant parents, LU26S (from Pakistan) and Kharchia (from India) along with a salt-sensitive cv. Potohar were grown in full strength Hoagland's nutrient solution with 0 or 125 mM NaCl. At the onset of the booting stage third leaf from top was sampled for protein analysis. Total soluble protein content increased due to salt treatment in all cultivars/lines but this increase was more marked in salt-sensitive cv. Potohar and low in salt-tolerant S24 as compared with the other lines. Patterns of labelled polypeptides in all cultivars/lines were identical; the differences were only quantitative (for instance, 29 kD and 48 kD polypeptides were reduced significantly due to NaCl treatment only in the cv. Potohar). This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:electrophoresis  polypeptides  SDS-polyacrylamide gel  salt stress  salt tolerance
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