Biological ballistics-no longer a shot in the dark |
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Authors: | Nigel P Batty Jean M Evans |
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Institution: | (1) Present address: Plant Breeding International (Cambridge) Ltd, Maris Lane, Trumpington, CB2 2LQ Cambridge, UK |
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Abstract: | Established methods of genetic transformation, such asAgrobacterium transfection and DNA uptake by protoplasts have not been successfully applied to some of the world’s major crops. This article
reviews the evolution of microprojectile bombardment, from its inception to establishment at the method of choice for transformation
of otherwise recalcitrant crops such as maize, wheat and barley. The potential of microprojectile bombardment, as a universal
method of transformation, is discussed in the context of the wide range of species transformed, together with the transformation
of plastid genomes and the contribution of this technology beyond the boundaries of the plant kingdom. |
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Keywords: | transformation microprojectile bombardment ballistics particle accelerator |
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