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| 利用小干扰RNA抑制小管间质性肾炎抗原相关蛋白的表达 | |
| 引用本文： | 杨智洪,王晓辉,姜艳超,李杰萍,袁斌,王朝云,杨树兴,叶棋浓.利用小干扰RNA抑制小管间质性肾炎抗原相关蛋白的表达[J].生物技术通讯,2008,19(2):185-187. |
| 作者姓名： | 杨智洪 王晓辉 姜艳超 李杰萍 袁斌 王朝云 杨树兴 叶棋浓 |
| 作者单位： | 1. 军事医学科学院,生物工程研究所,北京,100850 2. 沈阳农业大学,辽宁,沈阳,110161 3. 河北省承德市双桥区卫生局,河北,承德,067000 |
| 基金项目： | 国家自然科学基金 , 解放军医药卫生科研项目 |
| 摘 要： | 目的：设计并构建TIN-ag-RP基因的小干扰RNA（siRNA），检测其对小管间质性肾炎抗原相关蛋白（TIN—ag-RP）表达的干扰效果。方法：设计2条针对TIN-ag-RP基因的siRNA，并克隆到siRNA表达载体pSliencer2．1-U6 neo上；经酶切和测序证明构建成功后，将重组质粒和带FLAG标签的TIN-ag-RP基因共转染293T人胚肾细胞，通过Westernblot检验siRNA的干扰效果。结果：获得了2个TIN-og-RP基因siRNA真核表达载体，均能有效抑制TIN-ag-RP的表达，其中一条的抑制效率诂90％以上。结论：构肆的TIN-ag-RP某因的siRNA能有效抑制TIN-ag-RP的表达。 |
| 关 键 词： | 小管间质性肾炎抗原相关蛋白 小干扰RNA 基因表达 |
| 文章编号： | 1009-0002(2008)02-0185-03 |
| 修稿时间： | 2007年5月28日 |
Inhibition of the Expression of Tubulointerstitial Nephritis Antigen Related Protein by Small Interference RNA |
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| YANG Zhi-Hong,WANG Xiao-Hui,JIANG Yan-Chao,LI Jie-Ping,YUAN Bin,WANG Zhao-Yun,YANG Shu-Xing,YE Qi-Nong.Inhibition of the Expression of Tubulointerstitial Nephritis Antigen Related Protein by Small Interference RNA[J].Letters in Biotechnology,2008,19(2):185-187. | |
| Authors: | YANG Zhi-Hong WANG Xiao-Hui JIANG Yan-Chao LI Jie-Ping YUAN Bin WANG Zhao-Yun YANG Shu-Xing YE Qi-Nong |
| Institution: | YANG Zhi-Hong, WANG Xiao-Hui, JIANG Yan-Chao, LI Jie-Ping, YUAN Bin, WANG Zhao-Yun, YANG Shu-Xing, YE Qi-Nong( 1. Beijing Institute of Biotechnology, Beijing 100850; 2. Shenyang Agricultural University, Shenyang 110161; 3. Shuang Qiao Qu Health Bureau, Chengde 067000; China) |
| Abstract: | Objective： To design and construct the small interference RNA（siRNA） for TIN-ag-RP gene and investigate the interference effect on the expression of tubulointerstitial nephritis antigen related protein（TIN-ag-RP）. Methods： Two TIN-ag-RP siRNAs were designed and inserted into pSliencer 2.1-U6 neo expression vector. After confirmation of the constructs by restriction digestion and DNA sequencing, human embryo kidney 293T cells were cotransfected with the re- combination plasmids and FLAG-tagged TIN-ag-RP expression vector. The effect of TIN-ag-RP siRNAs on the expression of TIN-ag-RP was identified by Western blot. Results： The two TIN-ag-RP siRNAs were obtained and could effectively inhibit the expression of TIN-ag-RP, one of which could inhibit the expression up to 90% or more. Conclusion： The constructed TIN-ag-RP siRNAs could effectively inhibit the expression of TIN-ag-RP. |
| Keywords: | tubulointerstitial nephritis antigen related protein small interference RNA gene expression |
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