DNA Fingerprinting of Individual Species and Intergeneric and Interspecific Hybrids of the Genera Bos and Bison, Subfamily Bovinae |
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Authors: | V A Vasil'ev E P Steklenev E V Morozova S K Semyenova |
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Institution: | (1) Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334, Russia;(2) Askaniya-Nova Biosphere Reserve, Kherson Oblast, 75230, Ukraine |
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Abstract: | Genome fingerprinting with a hypervariable minisatellite sequence of phage M13 DNA was used to study the genetic variation in individual species of the genera Bosand Bison(subfamily Bovinae) and in their interspecific and intergeneric hybrids. DNA fingerprints were obtained for domestic cow Bos taurus primigenius, vatussy Bos taurus macroceros, banteng Bos javanicus, gaur Bos gaurus, wisent Bison bonasus, bison Bison bison, and for the interspecific and intergeneric hybrids. Compared with the original species, most hybrids showed a greater variation in number and size of hybridization fragments. An association was revealed between the number of hybridization fragments and degree of consanguinity of interspecific hybrids resulting from unique crossing of domestic cow and banteng. Pairwise similarity coefficients were calculated to construct a dendrogram of genetic similarity, which reflected the relationships between the parental species and hybrids varying in degree of consanguinity. The applicability of the method for identifying interspecific and intergeneric hybrids and for studying the consequences of hybridization in the subfamily Bovinae is discussed. |
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