Effects of successivein vitro andIn vivo passages on the virulence of the entomopathogenic fungus,Nomuraea rileyi |
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| Authors: | C M Ignoffo A H McIntosh C Garcia M Kroha J M Johnson |
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| Institution: | (1) Biological Control of Insects Research Laboratory, U.S. Department of Agriculture, Science and Education Agricultural Research Service, 65205 Columbia, Mo, USA |
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| Abstract: | There was no significant decrease or increase in the activity of conidia ofNomuraea rileyi after 12in vivo serial passages in larvae ofTrichoplusia ni (Hübner) or after 12in vitro serial passages on a semi-synthetic medium. The LC-50 at the start of the serial passage was 16.8±4.0 conidia/mm2; after 12 serial passages the LC-50 for thein vitro- andin vivo-produced conidia was 16.2±2.5 conidia/mm2 and 12.0±1.9 conidia/mm2, respectively.
Résumé Il n'y a pas d'augmentation ou de réduction significative dans l'activité des conidies deNomuraea rileyi après 12 passages en sériein vivo chez les larves deTrichoplusia ni (Hübner) ou après 12 passages en sériein vitro sur un milieu semi-synthétique. La concentration léthale 50 au début de ces passages était de 16,8±4,0 conidies/mm2, après 12 passages la CL 50 des conidies produitesin vitro etin vivo fut respectivement de 16,2±2,5 conidies/mm2 et de 12,0±1,9 conidies/mm2.
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